Methylamine stimulates the action of ricin toxin but inhibits that of diphtheria toxin.
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منابع مشابه
Inhibition of coated pit formation in Hep2 cells blocks the cytotoxicity of diphtheria toxin but not that of ricin toxin
It has been recently shown (Larkin, J. M., M. S. Brown, J. L. Goldstein, and R. G. W. Anderson, 1983, Cell, 33:273-285) that after a hypotonic shock followed by incubation in a K+-free medium, human fibroblasts arrest their coated pit formation and therefore arrest receptor-mediated endocytosis of low density lipoprotein. We have used this technique to study the endocytosis of transferrin, diph...
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We have found that cerulenin, an antibiotic that inhibits de novo fatty acid and cholesterol biosynthesis and fatty acylation of proteins, strongly inhibited the cytotoxicity of ricin, modeccin, Pseudomonas toxin, and diphtheria toxin in a brefeldin A (BFA)-resistant mutant of Vero cells (BER-40). The protective effect of cerulenin against ricin was also observed in two other BFA-resistant cell...
متن کاملMode of Action of Diphtheria Toxin
In a preceding paper it was shown that low concentrations of diphtheria toxin, in the presence of NAD 1 as an essential cofactor, inhibit the incorporation of amino acids into TCA-precipitable peptides in mammalian cell extracts (1). Collier (2) has demonstrated that this inhibitory action of the toxin is due to the specific inactivation of transferase II , one of the highly labile soluble enzy...
متن کاملDiphtheria toxin: mode of action and structure.
INTRODUCTION.... 54 OUTLINE OF THEPROBLEM. 54 INHIBITION OFPROTEIN SYNTHESIS ..56 Studies in Cell Culture ..56 Studies in Cell-Free Systems . 8 ADP-RIBOSYLATION OF EF-2 .59 Identification of the Reaction . ADPR is Covalently Attached . .60 The Role of Toxin is Catalytic . 1 Substrate Specificity ..6 Reversibility and Thermodynamics .62 Factors Affecting the Rate of the Forward Reaction 62 STRUC...
متن کاملStudies on the Mode of Action of Diphtheria Toxin
Extracts from HeLa cells treated with excess diphtheria toxin for several hours, until all protein synthesis has been arrested, are still able to stimulate the poly U-directed incorporation of phenylalanine into polypeptides at a moderate rate. Activity may be restored to normal levels or above by addition of a soluble enzyme fraction containing transferase II. Our results are in agreement with...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1981
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)69952-7